Increase in mitotic recombination in diploid cells of Aspergillus nidulans in response to ethidium bromide

Increase in mitotic recombination in diploid cells of Aspergillus nidulans in response to ethidium bromide

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Ethidium bromide (EB) is an intercalating inhibitor of topoisomerase trikes II and its activities are related to chemotherapeutic drugs used in anti-cancer treatments.EB promotes several genotoxic effects in exposed cells by stabilising the DNA-enzyme complex.The recombinagenic potential of EB was evaluated in our in vivo study by the loss of heterozygosity of nutritional markers in diploid Aspergillus nidulans cells through Homozygotization Index (HI).

A DNA repair mutation, uvsZ and a chromosome duplication DP (II-I) were introduced in the genome of tested cells to obtain a sensitive system for the recombinagenesis detection.EB-treated diploid cells had HI values significantly greater than the control at both concentrations (4.0 x 10-3 and 5.

0 x 10-3 mM).Results indicate that the intercalating agent Diecast Truck is potentially capable of inducing mitotic crossing-over in diploid A.nidulans cells.